This mouse Bmi1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptid
e between 235-268 amino acids from the C-terminal region of mouse Bmi1.
背景介紹
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcription
ally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remod
eling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably
changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of
RNF2/RING2 (By similarity).
Anti-Bmi1 Antibody (C-term)at 1:2000 dilution + H-4-Ⅱ-E whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/